This program on the superresolved optical microscopy (or nanoscopy) aims at simplifying the STED microscopy (for Stimulated Emission Depletion) method using microchip lasers. It focusses on a (...)
In this NANOTECHNOLOGIES & NANOSYSTEMS project funded by the French program ANR P2N we plan to demonstrate a new concept that aims at enhancing the limited resolution capabilities of optical microscopes by using a micro-chip laser that delivers simultaneously two wavelengths.
Using light sheet microscopy(SPIM) we plan to shown that the STED effect using our DPSS-lasers will drastically improve the axial resolution. Indeed, as shown on the schematics of the LSFM (...)
As a first step our aim is not to perform imaging, the excitation-depletion processes are induced in a uniform distribution of fluorescence emitters, i.e. a Coumarin 490 dye solution and the (...)
Background & general objectives Microchip based microlaser is a perfect choice for the conception of a STED microscopy system. Its sub nanosecond pulse duration is well adapted to the (...)
The classic mask producing a quasi donut shape for the stimulating wavelength is mandatory for confocal-STED microscopy. For STED-SPIM, the stimulating light sheet can be obtained in a different and simpler way by using a mask that produces directly a zero intensity light sheet instead of a zero intensity central spot.
Understanding the molecular mechanisms underlying the pathological manifestations and progression of Alzheimer’s disease (AD) is crucial for developing effective, targeted therapies for those afflicted with this devastating disease.
Recent studies suggest that astrocytes may play a major role in synaptic dysfunction.
The light shit experiments gave us the opportunity to further investigate the astrocytes morphological changes in a transgenic animal model of AD. For this purpose, we used clarified tissue samples.