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ANR Nanoscolas

In this NANOTECHNOLOGIES & NANOSYSTEMS project funded by the French program ANR P2N we plan to demonstrate a new concept that aims at enhancing the limited resolution capabilities of optical microscopes by using a micro-chip laser that delivers simultaneously two wavelengths.

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Le projet NANOSCOLAS (ANR-13-NANO-0004)

This program on the superresolved optical microscopy (or nanoscopy) aims at simplifying the STED microscopy (for Stimulated Emission Depletion) method using microchip lasers. It focusses on a (...)

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La microscopy à feuillet de lumière superésolutive (STED-SPIM)

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Using light sheet microscopy(SPIM) we plan to shown that the STED effect using our DPSS-lasers will drastically improve the axial resolution. Indeed, as shown on the schematics of the LSFM (...)

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STED-SPIM améliore la résolution et étend le champ de vue

As a first step our aim is not to perform imaging, the excitation-depletion processes are induced in a uniform distribution of fluorescence emitters, i.e. a Coumarin 490 dye solution and the (...)

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Le laser pour le STED simplifié

Our first setup is based on a DPSS self-Q-switched Nd:YAG laser from TEEM-Photonics delivering 7 μJ nanosecond pulses at a rate of 20 kHz, we also investigate a more powerful model (100µJ) but (...)

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Microlaser bicolore à haute fréquence pour le STED simplifié

Background & general objectives Microchip based microlaser is a perfect choice for the conception of a STED microscopy system. Its sub nanosecond pulse duration is well adapted to the (...)

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Les colorants pour le STED simplfié

A number of blue fluorophores have spectral properties matched to the wavelengths delivered by the bi-color laser(355 nm for excitation and 532 nm for stimulation) such as Coumarin 490 shown on (...)

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Les masques de phase pour le STED simplifié

For simple STED scanning microscopy we kept the two wavelengths merged along the whole optical path to preserve their intrinsic alignment. However, the STED beam is to display a dark center, (...)

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Optimisation des masques de phase pour le STED-SPIM simplifié

The classic mask producing a quasi donut shape for the stimulating wavelength is mandatory for confocal-STED microscopy. For STED-SPIM, the stimulating light sheet can be obtained in a different and simpler way by using a mask that produces directly a zero intensity light sheet instead of a zero intensity central spot.

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Les astrocytes et la maladie de Alzheimer

Understanding the molecular mechanisms underlying the pathological manifestations and progression of Alzheimer’s disease (AD) is crucial for developing effective, targeted therapies for those afflicted with this devastating disease.
Recent studies suggest that astrocytes may play a major role in synaptic dysfunction.
The light shit experiments gave us the opportunity to further investigate the astrocytes morphological changes in a transgenic animal model of AD. For this purpose, we used clarified tissue samples.

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Les objets pour le STED-SPIM simplfié

Nanometer sized fluospheres are used to check the spatial resolution enhancenement provided by the simplfied STED-SPIM method. Microfabicated objects and slices of mouse brain are also good candidates.

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