We designed our experiment to have the dynamical control and measurement of both mechanical and biochemical signals. On the one hand, we are developing active substrates based on magnetic micropillars embedded in a thin layer of soft material. On the other hand, we intend to read the biochemical response with FRET-based biosensors. Thanks to an alternating excitation scheme, it is possible to measure quantitatively and in real-time the FRET signal. We now aim at combining both mechanical stimulation and biochemical readout to screen quantitatively the mechanotransduction dictionary.
The trainee will work together with a PhD student and a postdoctoral fellow, the whole project being a collaboration with biologists from the Institut Albert Bonniot and physicists from the Institut Néel. This internship requires experimental skills and a particular interest for interdisciplinary work. A follow-up PhD project would be to test back the signal transduction by activating signalling nodes via opto -genetics and measuring the mechanical cell response.
Keywords : fluorescence live cell imaging, FRET, magnetic force, signal processing
Encadrant : Aurélie Dupont, LIPhy, Université J. Fourier, Grenoble
Collaboration : O. Destaing (I. Albert Bonniot), N. Dempsey (I. Néel), Grenoble
Contact : email@example.com, 04 76 63 58 16