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Les colorants pour le STED simplfié

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A number of blue fluorophores have spectral properties matched to the wavelengths delivered by the bi-color laser(355 nm for excitation and 532 nm for stimulation) such as Coumarin 490 shown on the figure.
.A compound of particular biological interest in this spectral range is the coenzyme NADH, since this endogenous molecule has essential roles in cell metabolism and is frequently imaged with 350 nm excitation.