This master project follows a PhD which allowed acquiring a unique expertise in confocal and non-linear optics imaging and characterization of the cellular network in bone and dentin. Although the network characteristics could be precisely evaluated, the typical spatial resolution that can be achieved using those techniques is 200 nm in the transverse direction. A better optical resolution (50 nm or less) is, thus, required to characterize the smallest porosity and obtain reliable quantitative measurements. This could, in principle, be achieved by tracking single fluorophores in diluted concentration within the porosity, similar to PALM/STORM super-resolution techniques. The trick to overcome the optical diffraction limit, is to use very small particles (quantum dots, nanoparticles) which displacement can be used to reconstruct their local environment with about 10nm accuracy.
The objectives of this internship are :
to define a protocol for SPT imaging using quantum dots and gold nanoparticles.
to establish the data processing tools framework for image reconstruction.
to validate a proof of concept for SPT imaging of porosity in dentin.
This study first relies on experimental work (sample preparation, SPT microscopy) combined with image treatment to analyze the multiscale porosity.
This project is at the Physics/Medicine interface and could suit a candidate with a background in Physics or Materials Science wishing to strengthen his/her knowledge in imaging with biomedical perspectives, or to a biologist with a strong background in microscopy wishing to develop new methods for the study of complex networks similar to neurons or vascular ones.
The project will be performed in close collaboration with the laboratory MSSMat of the Ecole Centrale Paris and the INSERM U1033 unit in Lyon.
Aurélien Gourrier, firstname.lastname@example.org, 0476514729
Aurélie Dupont, aurelie.dupont@univ-grenoble-alpes .fr, 0476635816