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Accueil > Équipes > Fluctuations, Régulations et Evolution des systèmes vivants > Thème de l’équipe

Jan Bednar

Regulation of transcription by remodeling factors

publié le , mis à jour le

In eukaryotic cells the genomic DNA is packaged into chromatin. The basic unit of chromatin, the nucleosome, comprises an octamer of core histones (around which 145 bp of DNA are wrapped) and histone H1, which associates at the DNA entry/exit site of the particle. The nucleosomal DNA is inaccessible to most of the DNA acting factors. In order to make this DNA accessible, the cell uses energy(ATP)-dependent nucleosome remodeling factors (e.g. RSC, SWI/SNF etc.) .

Our research activity is recently concentrated around this remodeling. Using approaches of structural (electron cryo-microscopy) and molecular biology, we try to understand, what the individual stages of the remodeling reaction are, what the structural intermediates of the nucleosome are and what factors are capable to modify the remodeling. The histone variants proved to be important players in the remodeling as well as the linker histone. Therefore we try to map the possible combinations of these factors and their impact on the chromatin remodeling, which on the other hand has an important role in majority of processes using DNA as a substrate – i.e. gene expression, DNA repair etc.