Our research activity is recently concentrated around this remodeling. Using approaches of structural (electron cryo-microscopy) and molecular biology, we try to understand, what the individual stages of the remodeling reaction are, what the structural intermediates of the nucleosome are and what factors are capable to modify the remodeling. The histone variants proved to be important players in the remodeling as well as the linker histone. Therefore we try to map the possible combinations of these factors and their impact on the chromatin remodeling, which on the other hand has an important role in majority of processes using DNA as a substrate – i.e. gene expression, DNA repair etc.