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STED-SPIM made simple

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The fluorescence light sheet microscopy (also called Selective Plane illumination microscopy : SPIM) illuminates the sample just where it is necessary : the plane of observation. It is the reference method for the microscopic observation of living species due to its low invasivity and toxicity. It can for example produce 3D microscopic images of embryos all long during their development.

Nevertheless the research of thin slices for a better resolution reduces the field of observation, this is a consequence of the optical laws for classical beams. We have resolved this problem by a selective extinction of the fluorescence by stimulated emission depletion (STED) using a bicolor laser developed at this occasion.

These results have been published in Optics Express.