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A fast confocal system for calcium imaging in brain slices

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In brain slices, resolving fast Ca2+ fluorescence signals from submicron structures is typically achieved using two-photon or confocal scanning microscopy, an approach that limits the number of scanned points.

In the current issue of The Journal of Biophotonics, researchers of the LIPhy and their industrial collaborators present a novel multiplexing confocal system that overcomes this limitation. This system, developed in our institute thanks to the funding of the “Federation pour la Recherche sur le Cerveau” and “Rotary France”, to record fluorescence Ca2+ signal from brain slices, is based on a custom-made spinning disk and an ultrafast CMOS camera. The system can resolve signals at more than 1 kHz from tens of thousand submicron neuronal structures, such as synaptic spines, embedded in the slice. Thanks to its unprecedented combination of temporal and spatial resolution with relatively simple implementation, this system will be used in the study of the kinetics of ion channels underlying physiological membrane potential and calcium signals.

Voir en ligne : A novel multisite confocal system for rapid Ca2+ imaging from submicron structures in brain slices. Filipis L, Ait Ouares K, Moreau P, Tanese D, Zampini V, Latini A, Bleau C, Bleau C, Graham J, Canepari M. J Biophotonics. 2018