Nos tutelles


 

Rechercher




Accueil > Coin des étudiants > Stages Prolongeable par une thèse. > Tous les stages.

Investigating the capture of circulating cells to the blood vessel wall

publié le

Blood vessels are coated with a thin and soft deformable layer called the glycocalyx, which governs the behaviour of circulating cells under flow. Circulating cells, such as immune cells and stem cells, are captured by the glycocalyx via specific interactions and then migrate through the vessel wall to reach sites of injury ; this process is also hijacked by cancer cells during metastasis. Understanding the biophysical and biochemical factors that regulate cell capture to the glycocalyx requires new methods from soft matter physics, along with chemistry and materials sciences.

This multi-disciplinary project, across physics, biology and chemistry, investigates the role of the mechanical properties of the glycocalyx (its softness and deformability) in the initial capture of circulating cells under flow.

Over the course of this Master M2 internship, you will be given the opportunity to :
• use established methods from surface chemistry to develop blood vessel glycocalyx mimetics and immune cell mimetics
• design, build and test the experimental setup (a microfluidic device) for laminar flow assays
• perform flow assays on glycocalyx mimetics for tracking of cell mimetics in 3D (x,y,z using optical microscopy) under flow
• use sophisticated data analysis methods for analysing bead trajectories,
• depending on your background, you will have the opportunity to develop routines to model data, or to culture living cells and study their rolling behaviours on the glycocalyx mimetics using flow assays.
Moreover, you will be able to develop interpersonal skills by working in a multidisciplinary project involving physicists, chemists and biologists.

Background and skills expected

The candidate should be motivated to work in an international and interdisciplinary research team. A solid background in physics, physical chemistry or engineering, and a vivid interest in biological and soft matter physics are expected.

Références :
1. Yago et al. J Cell Biol 2004, 166, 913-23
2. Couzon et al. Eur Biophys J 2009, 38, 1035-47
3. Richter et al. J Am Chem Soc 2007, 129, 5306-7
4. Christophis et al. Biophys J 2011, 101, 585-93

Laboratory :
Dynamics of Complex Fluids and Morphogenesis (DYFCOM),Laboratoire Interdisciplinaire de Physique (LIPhy), Université Grenoble Alpes

Supervisors :
Heather DAVIES, Delphine DÉBARRE & Lionel BUREAU

Contact :

Heather Davies (+33 (0)4 76 51 48 12) heather.davies@univ-grenoble-alpes.fr
crédit image : MIT news