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Home > Research > Talks & Conferences > Talks Given at LIPhy

Invited Talks

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These talks are given by invited speakers at LIPhy. The intended audience is the whole LIPhy. A large general introduction intended for non-specialist is usually provided.

Typical talk duration is around one hour and includes about 15 mn of questions. The talks are scheduled usually every monday at 2PM. The place is at the conference room, second floor. Access to the lab can be obtained through the secretaries.

Agenda

  • Monday 27 May 14:00-15:30 - Uwe Schlattner

    Imaging AMPK activation and cellular energy state with FRET sensors

    Résumé : AMP-activated protein kinase (AMPK) represents a key cell signaling hub that senses and regulates energy state. AMPK activation by increasing AMP and ADP concentrations involves a conformational switch within the heterotrimeric complex. This is exploited here for the construction of a synthetic sensor of cellular energetics and allosteric AMPK activation, AMPfret. Based on engineered AMPK fused to fluorescent proteins, the sensor allows direct, real-time readout of the AMPK conformational state by fluorescence resonance energy transfer (FRET). AMPfret faithfully and dynamically reports the binding of AMP and ADP to the AMPK γ-CBS sites, competed by Mg2+ free ATP. The FRET signal correlates with activation of AMPK by allosteric mechanisms and protection from dephosphorylation, attributed here to specific CBS sites, but does not require AMPK activation loop phosphorylation. Moreover, AMPfret is capable to report binding of pharmacological activators to both allosteric sites, CBS and α/β-ADaM, thus enabling activator screening. Cellular assays demonstrate that AMPfret is applicable in vivo for spatiotemporal analysis of energy state and allosteric AMPK activation.



    contact: Marco Canepari & Aurélie Dupont

    Lieu : LIPhy, conference room - 140 Avenue de la Physique 38402 Saint Martin d’Hères


  • Monday 17 June 14:00-15:30 - Annie Colin - ESPCI

    Coating of Yield Stress Fluids

    Résumé : We study the thickness of the coating that results from dipping a rod into bath of yield-stress liquid or after scrapping an yield stress fluid with an elastic wiper moved at a velocity V on a wetting surface. These geometries are widely used in cosmetic applications. In both cases we will show that the behavior is very different from that of a simple fluid.
    In the dip coating geometry, the coating thickness is observed to depend non-linearly on both geometrical conditions and fluid properties. When the yield-stress is large in comparison with the capillary pressure, the proximity of the stationary wall has a profound effect on the coating thickness. Strikingly, the thickness is found to be solely governed by the fluid flow inside the bath, which is obtained using the conservation of mass condition and the boundary conditions. A good agreement between experiments (thickness, velocity profile) and theory is obtained. These findings can be used to optimize the coating thickness in industrial applications.
    In the context of doctor blade geometry, we highlight a particular instability linked to the flow of fluid in the reservoir part.



    contact: Jean-Louis Barrat

    Lieu : LIPhy, conference room - 140 Avenue de la Physique 38402 Saint Martin d’Hères


  • Monday 24 June 14:00-15:30 - Alessandro Siria - ENS de Paris

    Alessandro Siria

    Résumé :



    contact: Banjamin Cross

    Lieu : LIPhy, conference room - 140 Avenue de la Physique 38402 Saint Martin d’Hères


  • Tuesday 17 September 14:00-15:30 - Paolo POLITI

    LIPHY Seminar

    Résumé :



    contact: Chaouqi Misbah

    Lieu : LIPhy, conference room - 140 Avenue de la Physique 38402 Saint Martin d’Hères


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